HIF1A and ATM were the only two validated genes. HIF1A, ATM, HSPA5, MAPK8, MAPK14, TLR4, and CREB1 were identified as hub genes among 3,144 DEGs and 74 f-DEGs. Results: GSEA revealed a statistically significant difference in ferroptosis in OSAS (FDR < 0.05). ROS, MDA, and GSH methods were used to detect CIH-induced ferroptosis and oxidative stress. ![]() Sprague-Dawley rats were randomly separated into normoxia and CIH groups. The hub genes were validated with RT-qPCR, IHC, and other datasets. The mechanism was investigated using GSEA and immune infiltration. The TCGA database was used to obtain the thyroid cancer (THCA) gene expression profile, and hub genes were analyzed for differential and survival analysis. GO, DO, KEGG, and GSEA enrichment were performed, a PPI network was constructed and hub genes were screened. Differentially expressed genes (DEGs) were screened using the R software and intersected with the ferroptosis database (FerrDb V2) to get ferroptosis-related DEGs (f-DEGs). Methods: OSAS-related datasets (GSE135917 and GSE38792) were obtained from the GEO. The mechanism of ferroptosis in OSAS disease progression remains unknown. CIH exacerbates ferroptosis, which is closely related to malignancies. Chronic intermittent hypoxia (CIH), sleep fragmentation, and increased pleural pressure are central mechanisms of OSAS complications. OSAS promotes hypertension, diabetes, and tumor growth through unknown means. ![]() 2Department of Radiology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Chinaīackground: By 2020, the prevalence of Obstructive Sleep Apnea Syndrome (OSAS) in the US has reached 26.1Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, China.Peijun Liu 1, Dong Zhao 1, Zhou Pan 1, Weihua Tang 2, Hao Chen 1 and Ke Hu 1 *
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